Injection of pan into the circulation of zebrafish embryos results in podocyte injury that is characterized by foot process effacement 70. Edema in zebrafish is detected and rated as different grades of swelling in. A zebrafish tale of parabiosis, podocytes, and proteinuria. The conserved paired nephron of larval zebrafish is an excellent model for.
Puromycin aminonucleoside pan is an antibiotic that inhibits protein. The aminonucleoside puromycin and experimental histamine h 2receptor antagonists ici 125,211 and ici 162,846 are able to produce proteinuria and glomerular pathology in rats that resemble human minimal change glomerular disease. It is a potent translational inhibitor in both prokaryotic and eukaryotic cells. Puromycin causes early chain termination during translation. The efficacy of puromycin and adriamycin for induction of. A, b representative fluorescence images of the proximal tubule epithelial cells circled structure in a dynamin. Triptolide also inhibited podocyte apoptosis in our zebrafish model and in vitro. This website uses cookies to ensure you get the best experience. Structure, properties, spectra, suppliers and links for. Depending on the cumulative dosage, puromycin aminonucleoside.
Blocking the translation of dnm mrna in zebrafish embryos causes proteinuria. Puromycin or adriamycininduced nephrosis are among the oldest and most widely used models of human nephrotic syndrome, particularly using rats due to their susceptibility to both compounds. In the pronephric glomerulus of larval zebrafish we demonstrate quantifiable loss of slit diaphragm integrity in a zebrafish model of puromycin aminonucleoside pa toxicity. In one such study, two novel candidate proteins, sos rasrho guanine. This antibiotic hinders the growth of bacteria, protozoa, algae and mammalian cells. Zebrafish as a model for kidney function and disease springerlink.
Rapid screening of glomerular slit diaphragm integrity in larval. The podocytes and renal glomeruli in zebrafish kidney are structurally, molecularly and functionally. Increased dynamin expression precedes proteinuria in glomerular. By continuing to use this site, you agree to the use of cookies.
Pdf effect of puromycin aminonucleoside on podocyte p. Podocyte developmental defects caused by adriamycin in. Sialic acid supplementation ameliorates puromycin aminonucleoside nephrosis in rats. Our results provide novel evidence for the clinical application of triptolide. Effect of puromycin aminonucleoside on podocyte pcadherin article pdf available in journal of the korean society of pediatric nephrology 172. Triptolide attenuates proteinuria and podocyte apoptosis via. Puromycin, dihydrochloride, cas 58582, is an aminonucleoside antibiotic that inhibits protein synthesis by blocking the translation step and causes premature release of nascent polypeptide chains. Podocalyxin regulates pronephritic glomerular development in zebra fish. Hey all, im trying to select fibroblasts after transduction with shrna to integrin a5 but i dont know why, my cells are not dying after treatment. Increased dynamin expression precedes proteinuria in. Also, in zebrafish embryos, we confirm that knocking down dynamin. It inhibits protein synthesis by disrupting peptide transfer on ribosomes causing premature chain termination during translation. Puromycin aminonucleoside nsc 3056 aminonucleoside.
Injection of the antibiotic, puromycin aminonucleoside pan, causes either a. As observed in rodent models, the defined single dose of puromycin used in the present study results in podocyte effacement with a concurrent loss of glomerular selectivity. Gadd45b mediates podocyte injury in zebrafish by activating the. Puromycin aminonucleoside nsc 3056 is the aminonucleoside portion of the antibiotic puromycin, and used in nephrosis animal models. Puromycin is an aminonucleoside antibiotic produced by the bacterium streptomyces alboniger. As a further advantage, zebrafish embryos are permeable to low molecular. Puromycin labeling does not allow protein synthesis to be. The zebrafish, danio rerio, is a relevant vertebrate model for biomedical research and translational studies because of its broad genetic conservation with humans. Zebrafishing for novel genes relevant to the glomerular. Here we present a functional screening assay based on the clearance of a 70kda fluorescent dextran in another vertebrate system that is rapid and low in cost. Puromycin aminonucleoside is a reversible inhibitor of dipeptidyl peptidase ii and cytosol alanyl aminopeptidase. Michael mcvoys lab contains the insert puromycin nacetyl transferase and is published in biotechniques. Experimental renal disease was produced in young rats by daily subcutaneous injections of 6dimethylamino purine, 3aminodribose aminonucleoside. Defects caused by adriamycin in zebrafish embryos and larvae.
Puromycin aminonucleoside an overview sciencedirect topics. Zebrafish danio rerio have been established as a model for. The amount of puromycinlabeled peptides is then determined by using antipuromycin antibodies and immunoblot, facs, or fluorescent microscopy, and is. The physiologic, biochemical, and light microscopic changes were similar to those observed in human nephrosis.
1175 71 794 312 381 1383 1452 535 897 861 107 973 1089 537 668 1348 178 1462 207 1421 817 413 1489 23 676 1312 953 76 1383 570 1170 51 144 117 785